Transposon induced mutation in Gluconobacter oxydans with special reference to its direct-glucose oxidation metabolism1

Gluconobacter oxydans: its biotechnological applications.

Gluconobacter oxydans is a gram-negative bacterium belonging to the family Acetobacteraceae. G. oxydans is an obligate aerobe, having a respiratory type of metabolism using oxygen as the terminal electron acceptor. Gluconobacter strains flourish in sugary niches e.g. ripe grapes, apples, dates, garden soil, baker's soil, honeybees, fruit, cider, beer, wine. Gluconobacter strains are non-pathoge...

7 Regioselective Oxidation of Aminosorbitol with Gluconobacter oxydans, Key Reaction in the Industrial 1-Deoxynojirimycin Synthesis

Polyol Dehydrogenases of Gluconobacter Oxydans.

The secondary hydroxyl group involved in the oxidation, and the cis-vicinal secondary hydroxyl group, must have a D configuration jvith respect to t,he primary alcohol group adjacent to the site of oxidation. This specific mode of otida,tion facilitated the synthesis of several new ketoses. In a series of papers, Hudson and Richtmyer and their a.ssociates described the microbiological synthesis...

Biotransformation of patulin by Gluconobacter oxydans.

A bacterium isolated from patulin-contaminated apples was capable of degrading patulin to a less-toxic compound, ascladiol. The bacterium was identified as Gluconobacter oxydans by 16S rRNA gene sequencing, whereas ascladiol was identified by liquid chromatography-tandem mass spectrometry and proton and carbon nuclear magnetic resonance. Degradation of up to 96% of patulin was observed in apple...

13C Tracers for Glucose Degrading Pathway Discrimination in Gluconobacter oxydans 621H

Gluconobacter oxydans 621H is used as an industrial production organism due to its exceptional ability to incompletely oxidize a great variety of carbohydrates in the periplasm. With glucose as the carbon source, up to 90% of the initial concentration is oxidized periplasmatically to gluconate and ketogluconates. Growth on glucose is biphasic and intracellular sugar catabolism proceeds via the ...